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KMID : 0525720130180010019
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Journal of Chitin and Chitosan
2013 Volume.18 No. 1 p.19 ~ p.25
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Diethyl-aminoethylation of Low Molecular Weight Chitooligosaccharide Enhances Inducement of Apoptosis in Human Adenocarcinoma Cells
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Karagozlu Mustafa Zafer
Karadeniz Fatih
Kim Se-Kwon
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Abstract
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Chitooligosaccharides are the degraded oligomer of chitosan which have various biological functions. Researchers have proved that chemical modifications of chitooligosaccharides (COS) could enhance their bioactivities. In this study, potential apoptotic activities of modified (diethyl-aminoethly chitoligosaccharide, DEAE-COS) and non-modified COS were evaluated and compared using AGS human gastric cancer cells. DEAE-COS was prepared with chemical synthesized by addition of diethyl-aminoethyl group to COS (< 1 kDa) and confirmed by IR spectra results in comparison to previous study. AGS cell viability was measured after chitosan oligomer treatment for 24 and 48 h. Inhibition of cell proliferation on AGS cells due to DEAE-COS and COS treatment showed by dose dependent manner. Furthermore changes in nuclear morphology and changes in cell morphology were observed by light and fluorescent microscopy. Moreover, protein and gene expressions of important regulators involved in apoptosis pathway such as Caspase-9, Bax, p53 and p21 were examined using RT-PCR and Western blot analysis. In compare analysis, even DEAE-COS and COS inhibited apoptosis on AGS cell, DEAE-COS showed significantly higher apoptotic activity than COS. The present results suggest that diethyl-aminoethly addition to low molecular weight COS enhances its apoptotic activity on AGS human adenocarcinoma cells.
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KEYWORD
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Chitooligosaccharide, Diethyl-aminoethyl, Apoptosis, AGS
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